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Today [experiment 2 begins]

Left home at 7:45 am, visited the ATM, then sat in a courtyard on campus checking e-mail and starting to read an article for lab meeting at noon. At 8:45, I headed over to Monterey Market for groceries, then made it back to lab at 9:40 for a game-day meeting with the undergrad co-leading the project. We assembled a setup plan, then ran around getting stuff organized until 11:30, at which point we switched gears to read the rest of the article for lab meeting. Lab meeting from 12-1 or so. More running around, mixing of solutions, labeling of tubes, setup of the respirator, acquiring of liquid nitrogen. At 3:45 we realized we'd forgotten to remove food from the crickets at noon, so plans to get underway at 4 were scrapped and new plans were made to start with the 9 pm timeslot. I switched over to the feeding experiment and wrapped that up at 6:30, so enough time to go grab some gross slices of pizza before we get underway again at around 7:30. I anticipate I'll get home at 11 pm.

Tomorrow we'll aim for the 1 pm and 5 pm timeslots, which means I'll need to get to the lab at 8 to remove food from the 1 pm crickets (they need to be post-absorptive for 4 hours before experiments begin).

I did a preliminary analysis of the lipid content of the hemolymph yesterday. The long-winged crickets have much higher lipids in their hemolymph shortly before lights-off, whereas the short-winged crickets have lower constituitive levels and no difference at different times of day.

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Comments

( 2 remarks — Remark )
randomdreams
Jul. 6th, 2016 03:44 am (UTC)
That sounds like an incredibly busy day.
rebeccmeister
Jul. 6th, 2016 05:50 pm (UTC)
No joke. It's going to take a little while to get into the swing of things, although today is looking less hectic, mercifully.
( 2 remarks — Remark )

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